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KMID : 0363020100400060265
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Journal of Korean Academy of Periodontology
2010 Volume.40 No. 6 p.265 ~ p.270
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Immunomodulatory effect of canine periodontal ligament stem cells on allogenic and xenogenic peripheral blood mononuclear cells
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Kim Hak-Sung
Kim Kyoung-Hwa
Kim Su-Hwan
Kim Young-Sung
Koo Ki-Tae
Kim Tae-Il
Seol Yang-Jo
Ku Young
Rhyu In-Chul
Chung Chong-Pyoung
Lee Yong-Moo
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Abstract
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Purpose: The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro.
Methods: Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells) and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established following the addition of phytohemagglutinin. The proliferation of PBMCs was evaluated using the MTS assay. The cell division of PBMCs was analyzed using the CFSE assay. The apoptosis of PBMCs was assessed using the trypan blue uptake method.
Results: Periodontal ligament stem cells and bone marrow stem cells inhibited the proliferation of allogenic and xenogenic PBMCs. Both periodontal ligament stem cells and bone marrow stem cells suppressed the cell division of PBMCs despite the existence of a mitogen. No significant differences in the percentages of apoptotic PBMCs were found among the groups.
Conclusions: Canine periodontal ligament stem cells have an immunomodulatory effect on allogenic and xenogenic PBMCs. This effect is not a product of apoptosis of PBMCs but is caused by the inhibition of cell division of PBMCs.
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KEYWORD
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Allogenic, Immunomodulation, Periodontal ligaments, Stem cell, Xenogenic
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